X 100 → Formate

HRID 743966Notes

Reaction equation

[C:1]([O-:5])(=[O:4])[CH2:2][OH:3].CCC(C[O:12]C(C(N(CC[NH+](C)C)C)=O)(C1C=CC=CC=1)C1C=CC=CC=1)CC.[Cl-]>>[C:1]([O-:5])(=[O:4])[CH:2]=[O:3].[CH:1]([O-:5])=[O:4].[C:2]([O-:12])(=[O:3])[C:1]([O-:5])=[O:4]

Participants

Reactant 2

HCID 19153X 100

HCID 5460308Hydroxyacetate

Product 3

HCID 283Formate

HCID 71081Oxalate

HCID 3614358Npc324351

Procedure

The reaction in Example 9 was repeated, substituting 0.75 g of a Pichia pastoris transformant MSP10 (13.2 IU glycolate oxidase and 21,200 IU catalase) which had been permeabilized by treatment with 0.1% Triton X-100/1 freeze-thaw for the Hansenula polymorpha transformant. After 16 h, the HPLC yields of glyoxylate, formate, and oxalate were 30.5%, 59.2%, and 10.7%, respectively, and 0.8% glycolate remained.

Workup

customThe reaction in Example 9

Sources & evidence

ORD record: ord-0b830ffcf39b44c28f4e6723d6706994
Source dataset: uspto-grants-1996_09
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US05559020

Reaction notes

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