Glycolic Acid → Formate

Participants

Reaction Steps

The reaction in Example 12 was repeated., using a mixture containing glycolic acid (0.500M), AMPA (0.525M), isobutyric acid (0.100M, HPLC internal standard), flavin mononucleotide (0.01 mM), and 0.72 g of Hansenula polymorpha transformant G01 (43.0 IU glycolate oxidase and 39,880 IU catalase) which had been permeabilized by treatment with 0.2% benzalkonium chloride (Lonza Barquat OJ-50) at pH 8.3 (adjusted with 50% NaOH) and 5° C. After 1 h, the HPLC yields of glyoxylate, formate, and oxalate were 50.2%, 47.4%, and 1.1%, respectively, and 2.2% glycolate remained. The remaining permeabilized cell activity of glycolate oxidase and catalase were 95% and 105%, respectively, of their initial values.

Provenance & Evidence