Hydrochloric Acid → HCID 67746223, compound

Participants

Reaction Steps

1.5 g of ethyl [[(S)-3-[(S)-1-(amino-imino-methyl)-piperidin-3-ylmethylcarbamoyl]-2-benzyloxycarbonylamino-propionyl]-cyclopropyl-amino]-acetate hydrochloride (Example 1) are dissolved in 15 ml of THF, treated with 7.9 ml of 1N lithium hydroxide solution and stirred at room temperature for 90 minutes. Then, the solution is treated with 7.9 ml of 1N hydrochloric acid and evaporated. The residue is chromatographed on RP-18 with a water-acetonitrile gradient. 0.63 g of colourless [[(S)-3-[(S)-1-(amino-imino-methyl)-piperidin-3-ylmethylcarbamoyl]-2-benzyloxycarbonylamino-propionyl]-cyclopropylamino]-acetic acid, MS (ion spray): 503.5 (M+H)+, is obtained.

Provenance & Evidence