反应详情

42 mg (0.11 mmol) 6-[4-(7-methoxy-2-oxo-1,2,4,5-tetrahydro-1,3-benzodiazepin-3-yl)-piperidin-1-yl]pyrimidine-4-carboxylic acid and 25 mg (0.11 mmol) 1′-methyl-2′,3′-dihydro-1′H-spiro[cyclopentan-1,4′-isoquinoline hydrochloride in 63 μL (0.36 mmol) DIPEA and 1.0 mL DMF were combined with 39 mg (0.12 mmol) TBTU and the mixture was stirred overnight at RT. Then the reaction mixture was purified by preparative HPLC-MS. The product-containing fractions were combined and partially evaporated down. The precipitate formed was suction filtered and dried. The filtrate was freeze-dried. The residue remaining was identical to the precipitate.

1. customThen the reaction mixture was purified by preparative HPLC-MS
2. custompartially evaporated down
3. customThe precipitate formed
4. filtrationfiltered
5. customdried
6. customThe filtrate was freeze-dried