Sodium Carbonate → HCID 178476630, compound

Participants

Reaction Steps

A mixture of N-(1H-3-indazolyl)-N-[4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl]amine (0.064 g, 0.000191 mol), trans-3-iodo-1-[4-(4-methylpiperazino)-cyclohexyl]-1H-pyrazolo[3,4-d]pyrimidin-4-amine (0.070 g, 0.000159 mol), tetrakis-(triphenylphosphine)palladium (0.011 g, 0.0000095 mol) and sodium carbonate (0.042 g, 0.000398 mol) was heated in a mixture of ethylene glycol dimethyl ether (4 mL) and water (2 mL) at 80° C. for 16 hours under an atmosphere of nitrogen. The mixture was allowed to cool to ambient temperature and solvents were removed under the reduced pressure. The residue was purified by preparative HPLC (Hypersil C18, 8 μm, 25 cm; 10-60% acetonitrile-0.1M ammonium acetate over 25 min, 21 mL/min) to yield trans-3-[4-(1H-3-indazolylamino)phenyl]-1-[4-(4-methylpiperazino)cyclohexyl]-1H-pyrazolo[3,4-d]pyrimidin-4-amine acetate (0.035 g, 0.000060 mol) as a white solid. 1H NMR (DMSO-d6, 400 MHz) δ 12.09 (s, 1H), 9.14 (s, 1H), 8.21 (s, 1H), 7.99 (d, 1H), 7.83 (d, 2H), 7.55 (d, 2H), 7.37 (m, 2H), 7.06 (t, 1H), 4.64 (m, 1H), 2.6-2.2 (br, 9H), 2.13 (s, 3H), 2.05 (m, 6H), 1.91 (s, 3H), 1.49 (m, 2H); RP-HPCL (Delta Pak C18, 5 μm, 300 A, 15 cm; 5%-85% acetonitrile−0.1M ammonium acetate over 20 min, 1 mL/min) Rt 12.96 min. MS: MH+ 523.

Provenance & Evidence