Hydrochloric Acid → HCID 69155979, compound

Participants

Reaction Steps

To a mixture of [4-(6-bromo-1-oxo-4-phenyl-3-propionyl-1H-isoquinolin-2-ylmethyl)benzenesulfonylamino]acetic acid methyl ester (70 mg), methanol (2.0 ml) and THF (2.0 ml) was added 1N sodium hydroxide (0.23 ml) at room temperature with stirring, and the mixture was stirred at the same temperature for 18 hrs. The reaction mixture was neutralized with 1N hydrochloric acid and concentrated under reduced pressure. The residue was purified by preparative HPLC to give the title compound (33 mg) in beige crystals. 1H NMR (CDCl3) δ: 0.52 (3H, t, J=6.9 Hz), 1.78 (2H, q, J=6.9 Hz), 3.82 (2H, d, J=6.0 Hz), 5.25-5.36 (3H, m), 7.23-7.31 (2H, m), 7.33 (2H, d, J=8.4 Hz), 7.44-7.55 (4H, m), 7.70 (1H, dd, J=1.8, 8.4 Hz), 7.80 (2H, d, J=8.4 Hz), 8.37 (1H, d, J=8.4 Hz). HPLC analysis: purity 95.2% (retention time: 4.38 min). MS (ESI+): 583 (M+H), 585.

Provenance & Evidence