2-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid → Fluorescein Methotrexate

Participants

Reaction Steps

2.5-5×105 cells were incubated over-night at 29° C. in 0.5 mL of culture medium containing 10 mM fluorescein-labeled methotrexate (F-MTX, Molecular Probes/Invitrogen). Labeled cells were washed in serum-free culture medium and images were recorded using a fluorescence microscope (Olympus CKX41 microscope equipped with a DP50 digital camera) using a FITC filter set.

Provenance & Evidence