反应详情

Resveratrol (Indofine #024964) was dissolved in Ethanol or DMSO to a 10 mM stock solution. Resveratrol was added to 60 mm NGM agar dishes containing either OP50 or RNAi expressing bacteria (HT115) to a final concentration of 10 μm, 50 μm, and 100 μM. Nile Red was also added to plates to a final concentration of 0.05 μg/ml. Nicotinamide (Supelco #47865-U) was diluted in PBS including Nile Red and added to 60 mM dishes containing OP50 to a final concentration of 1 mM, 10 mM, or 100 mM.

1. additioncontaining either OP50 or RNAi
2. additionadded to 60 mM dishes
3. additioncontaining OP50 to a final concentration of 1 mM, 10 mM, or 100 mM