Sodium Chloride → HCID 178511805, compound

HRID 840044Notes

Reaction equation

HRID 840044 reaction equation
[NH2:1][C@H:2]([C:9]([OH:11])=[O:10])[CH2:3][C:4]1[N:8]=[CH:7][NH:6][CH:5]=1.[Cl-:12].[Na+:13]>O>[NH2:1][C@H:2]([C:9]([OH:11])=[O:10])[CH2:3][C:4]1[N:8]=[CH:7][NH:6][CH:5]=1.[Cl-:12].[Na+:13]

Participants

4

Reactant 2

HCID 5234Sodium ChlorideHCID 178419078HCID 178419078, compound

Product 1

HCID 178511805HCID 178511805, compound

Reagents, catalysts & solvents 1

HCID 962WaterSolvent

Conditions

Temperature
25 °C

Procedure

Ethanol was removed from the liposome suspension by diafiltration. A histidine/sodium chloride solution was prepared by dissolving histidine (10 mM) and sodium chloride (150 mM) in sterile water. The pH of the solution was adjusted to approximately 7. The solution was filtered through a 0.22 μm Durapore filter. The liposome suspension was diluted in approximately a 1:1 (v/v) ratio with the histidine/sodium chloride solution and diafiltered through a polysulfone hollow-fiber ultrafilter. Eight volume exchanges were performed against the histidine/sodium chloride solution to remove the ethanol. The process fluid temperature was maintained at about 20-30° C. Total diafiltration time was approximately 4.5 hours.

Workup

  1. customEthanol was removed from the liposome suspension by diafiltration
  2. filtrationThe solution was filtered through a 0.22 μm
  3. filtrationDurapore filter
  4. additionThe liposome suspension was diluted in approximately a 1:1 (v/v) ratio with the histidine/sodium chloride solution
  5. customto remove the ethanol

Sources & evidence

ORD record
ord-a5456307292344baa080cfe726f5ee15
Source dataset
uspto-grants-2002_04
Patent number
US06365179B1

Reaction notes

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