HuagongsheHuagongshe Chemistry Print Report · For research reference only
Trisodium citrate → FR-179642
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | Trisodium citrate | HCID 6224 | — |
| Reactant | Trichloroacetic Acid | HCID 6421 | — |
| Reactant | [5-[(1S,2S)-2-[(3S,6S,9S,11R,15S,18S,20R,21S,24S,25S,26S)-3-[(1R)-3-amino-1-hydroxy-3-oxopropyl]-18-(hexadecanoylamino)-11,20,21,25-tetrahydroxy-15-[(1R)-1-hydroxyethyl]-26-methyl-2,5,8,14,17,23-hexaoxo-1,4,7,13,16,22-hexazatricyclo[22.3.0.09,13]heptacosan-6-yl]-1,2-dihydroxyethyl]-2-hydroxyphenyl] hydrogen sulfate | HCID 44352613 | — |
| Product | FR-179642 | HCID 71714631 | — |
Reaction Steps
To 7 ml of the fermentation broth of Oidiodendron sp. No. 30084 obtained in Example 1-1 was added 1 ml of an aqueous solution (20 ml/ml) of FR901379 Substance (20 mg as FR901379 Substance; 16.7 μmol) as well as 2 ml of a buffer (0.2 M sodium citrate buffer, pH 4.0), and the reaction was carried out at 30° C. for 3 hours. The reaction was then stopped by adding 0.4 M trichloroacetic acid and centrifuged at low speed to remove precipitated high molecular weight protein and other impurities. The FR179642 Substance produced was subjected to HPLC and monitored at 210 nm to determine the acylase activity.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US06372474B1 |
| Source dataset | uspto-grants-2002_04 |
| ORD ID | ord-cdf022789ef24ea887dfb522d0064deb |
| Source note | uspto-grants-2002_04 |