HCID 14096709, compound → HCID 178501392, compound

Participants

Reaction Steps

To the solution containing 15.84 g (25 mmoles) of Glp-His(DNP)-Trp-OMe protected tripeptide in 100 ml of DMF and 40 ml of water, 4 ml of mercaptoethanol are added, then the pH-value of the solution is adjusted to 8 by adding TEA. The solution is left to stand at room temperature for 30 minutes, then evaporated to dryness under reduced pressure. The oily residue is thoroughly triturated with ether, filtered and dried. The thus-obtained product is dissolved in a little volume of methanol and crystallized by adding ether. The crystals are filtered and dried to give 10.92 g (93.5% yield) of the aimed compound, m.p.: 228°-232° C.

Provenance & Evidence