Butyric Acid → Formate

Participants

Reaction Steps

The reaction in Example 7 was repeated, using an aqueous solution containing glycolic acid (0.50M), aminomethylphosphonic acid (AMPA, 0.40M), FMN (0.01 mM), butyric acid (HPLC internal standard, 0.10M), glycolate oxidase (from spinach, 1.0 IU/mL), and catalase (from Aspergillus niger, 14,000 IU/mL) at pH 8.5. After 17.5 h, the HPLC yields of glyoxylate, formate, and oxalate were 91.0%, 2.9%, and 2.9%, respectively, and 4.1% glycolate remained. The remaining activity of glycolate oxidase and catalase were 63% and 91%, respectively, of their initial values.

Provenance & Evidence