Butyric Acid → Formate

Participants

Reaction Steps

The reaction in Example 7 was repeated, using an aqueous solution containing glycolic acid (1.0M), aminomethylphosphonic acid (AMPA, 0.80M), FMN (0.01 mM), butyric acid (HPLC internal standard, 0.01M), glycolate oxidase (from spinach, 2.0 IU/mL), and catalase (from Aspergillus niger, 14,000 IU/mL) at pH 8.5.. After 66 h, the HPLC yields of glyoxylate, formate, and oxalate were 78.9%, 2.2%, and 12.1%, respectively, and 2.0% glycolate remained. The remaining activity of glycolate oxidase and catalase were 64% and 87%, respectively, of their initial values.

Provenance & Evidence