MES NaOH → (2R,3S,4R,5R)-2-(hydroxymethyl)-5-(6-hydroxypurin-9-yl)oxolane-3,4-diol

Participants

Reaction Steps

The phosphomonoesterase activity was measured under the following condition using 5'-inosinic acid as a substrate. The reaction was performed at 30° C. for 10 minutes in a reaction solution (1 ml) containing 10 μmol/ml of 5'-inosinic acid, 100 μmol/ml of MES/NaOH buffer (pH 6.0), and an enzyme. The reaction was stopped by adding 200 μl of 2 N hydrochloric acid. After that, precipitates were removed by centrifugation. Then, inosine produced by the hydrolytic reaction was quantitatively measured. An amount of enzyme to produce 1 μmol of inosine per 1 minute under this standard reaction condition was defined as 1 unit.

Provenance & Evidence