L-Tyrosine → HCID 13146039, compound

Participants

Reaction Steps

To 0.50 g of BOC-Tyr-(D)-Met(O)-Gly-EtPhe-NHNHCOCH2CH3 were added 0.5 ml of anisole and 5 ml of TFA, and the mixture was shaken for 10 minutes. The solvent was distilled off, and ether was added to recover by filtration the product in the form of powder. The product was subjected to ion exchange on Amberlite IRA 410 (acetic acid type), and then poured on a column (2.2×120 cm) of Cephadex LH-20. The elution was conducted with 0.1 N acetic acid, and the fractions of 300 to 350 ml were collected and lyophilized. Yield: 0.35 g [α]D24 +18.5° (c=0.49, MeOH), Rf2, 0.24. Amino Acid analysis (4% thioglycolic acid/6 N hydrochloric acid hydrolysis): Gly 1.00(1), Met 1.05(1), Tyr 0.97(1).

Provenance & Evidence