N2 {N2, N6-Bis[3-(2,3,4,6-tetra-O-acetyl-alpha-D-mannopyranosylthio)propionyl]-L-lysyl}-N6-[3-(2,3,4,6-tetra-O-acetyl-alpha-D-mannopyranosylthio)propionyl]-L-lysine → N2 {N2,N6-Bis-[3-(alpha-D-mannopyranosylthio)propionyl]-L-lysyl}-N6-[3-(alpha-D-mannopyranosylthio)propionyl]-L-lysine

Participants

Reaction Steps

A solution of 4 (2.0 g) in methanol-water-triethylamine 5:4:1 (v/v/v, 20 ml) is kept overnight at room temperature, and evaporated in vacuo to a residue that is put on a column of silica gel and eluted with chloroform-methanol-water 60:40:10 (v/v/v). The desired fractions are combined and evaporated to dryness. A solution of this compound in water (15 ml) is lyophilized to give 5 (1.2 g, 90%): mp 95°-100° C.; [α]D27 +115±0.9° (c 1.1, H2O); NMR (D2O) δ: 5.37 (s, 1H, H-1), 5.34 (s, 2H, H-1), 4.36, 4.19 (q,q, α-CH), 3.24 (m, ε-CH2), 2.95 (m, SCH2), 2.71 (t, 2H), 2.61 (t, 4H) (SCH2CH2), 1.33-1.92 (CCH2CH2CH2C).

Provenance & Evidence