Phosphate → 3-Hydroxy-5-phenoxypentanoic acid

HRID 859819Notes

Reaction equation

P([O-])([O-])([O-])=[O:2].[OH:6][C@H:7]([CH2:59][CH2:60][O:61][C:62]1[CH:67]=[CH:66][CH:65]=[CH:64][CH:63]=1)[CH2:8][C:9](SCCNC(=O)CCNC(=O)[C@H](O)C(C)(C)COP(O)(=O)OP(O)(=O)OC[C@H]1O[C@@H](N2C3N=CN=C(N)C=3N=C2)[C@H](O)[C@@H]1OP(O)(O)=O)=[O:10]>>[OH:6][CH:7]([CH2:59][CH2:60][O:61][C:62]1[CH:67]=[CH:66][CH:65]=[CH:64][CH:63]=1)[CH2:8][C:9]([OH:10])=[O:2]

Participants

Reactant 2

HCID 1061Phosphate

HCID 86588298(R)-3-hydroxy-5-phenoxyvaleryl CoA

Product 1

HCID 220240393-Hydroxy-5-phenoxypentanoic acid

Procedure

Then, the pellet was suspended in 480 parts of a 0.1M phosphate buffer (pH 7.0), and 5 parts of (R)-3-hydroxy-5-phenoxyvaleryl CoA (prepared by a method described in Eur. J. Biochem., 250, 432–439 (1997) after obtaining 3-hydroxy-5-phenoxyvaleric acid by hydrolyzing 3-hydroxy-5-phenoxylvaleric acid ester obtained by a Reformatsky reaction using zinc, in which 3-phenoxypropanal synthesized by a method described in J. Org. Chem., 55, 1490–1492 (1990) and ethyl bromoacetate were used as raw materials) and one part of bovine serum albumin (Sigma) were added to the suspension before moderately shaking the suspension for 30 minutes at 30° C.

Workup

customprepared by a method

Sources & evidence

ORD record: ord-9840506b722147eb971752760676e51e
Source dataset: uspto-grants-2006_12
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US07153622B2

Reaction notes

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