Hydrochloric Acid → HCID 14151451, compound

Participants

Reaction Steps

In 50 ml of water were dissolved 2.4 g of α-L-aspartyl-L-phenylalanine methyl ester and 0.7 g of sodium bicarbonate. A solution of 1.9 g of N-benzyloxycarbonyl-D-alanine N-hydroxysuccinimide ester in 50 ml of dioxane was added to the solution. The mixture was stirred at room temperature for 5 hours. After the pH was adjusted to 2.5 with 6N hydrochloric acid, 150 ml of ethyl acetate was added to the mixture. The separated ethyl acetate layer was wahsed with water and a saturated sodium chloride aqueous solution and dried over anhydrous sodium sulfate. Sodium sulfated was removed, and ethyl acetate was distilled off under reduced pressure. The residue was reprecipitated from ethyl acetate/hexane. Yield 2.1 g.

Provenance & Evidence