反应详情

In a brown flask, 4-chloro-7-nitro-2,1,3-benzoxadiazole (NBD-Cl) (483 mg) was placed and dissolved in methanol (14.0 ml). Under an argon atmosphere, a solution in which L-glucosamine hydrochloride (283 mg) was dissolved in 0.3 M NaHCO3 solution (5.57 ml) was added thereto, and the resulting mixture was stirred at room temperature. After 24 hours, methanol was removed by concentration under reduced pressure, and the resulting precipitates (unnecessary substances derived from NBD-Cl) were removed by filtration. The precipitates were washed with water, and the filtrate and the washing solution were combined, followed by purification by HPLC. A target fraction was collected and lyophilized, whereby a target product was obtained (yield: 102 mg, yield ratio: 23%).

1. additionwas added
2. custommethanol was removed by concentration under reduced pressure
3. customthe resulting precipitates (unnecessary substances derived from NBD-Cl)
4. customwere removed by filtration
5. washThe precipitates were washed with water
6. customfollowed by purification by HPLC
7. customA target fraction was collected
8. customwhereby a target product was obtained
9. custom(yield: 102 mg, yield ratio: 23%)