Hydrochloric Acid → HCID 56644347, compound

Participants

Reaction Steps

18 mg (0.04 mmol) {1-[4-(3-Methyl-7-phenyl-imidazo[1,2-b]pyridazin-6-yl)-phenyl]-cyclobutyl}-carbamic acid tert.-butyl ester (intermediate example Int-3-0) were dissolved in 01 mL dioxane. 0.4 mL 4M hydrogen chloride in dioxane were added and the mixture was stirred over night at room temperature. The solvent was evaporated and the residue was treated with saturated sodium bicarbonate solution. After stirring for one hour the reaction mixture was extracted three times with ethyl acetate. The combined organic extracts were washed with water and dried (sodium sulfate). After removal of the solvent the residue was purified by chromatography on silicagel (eluents dichloromethane/methanol) and additional HPLC yielding 2.3 mg (14%) of the title compound.

Provenance & Evidence