Sodium Hydroxide → 3-{2-[(Cyclopentyl{4-[(5-oxo-2-phenyl-5,6-dihydro-4H-1,3,4-oxadiazin-4-yl)methyl]phenyl}acetyl)amino]-1,3-thiazol-4-yl}propanoic acid

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Reaction Steps

20 mg (0.1 mmol) of ethyl 3-(2-amino-1,3-thiazol-4-yl)propanoate [Beilstein Reg. No. 9762098], 41.7 mg (0.13 mmol) of TBTU and 25.8 mg of ethyldiisopropylamine were added to a solution of 39.2 mg (0.1 mmol) of cyclopentyl{4-[(5-oxo-2-phenyl-5,6-dihydro-4H-1,3,4-oxadiazin-4-yl)methyl]phenyl}acetic acid (Example 51A) in 0.5 ml DMSO. The mixture was stirred at room temperature overnight. 0.3 ml of 2 M aqueous sodium hydroxide solution was then added, and the mixture was once more stirred overnight. The solvent was then evaporated, and the residue obtained was purified directly by preparative HPLC/MS. This gave 7.1 mg (0.013 mmol, 13% of theory) of the title compound.

Provenance & Evidence