Diisopropylethylamine → ethyl(4-{(S)-{[(4-tert-butylphenyl)acetyl]amino}[5-(2,2,2-trifluoroethoxy)pyridin-2-yl]methyl}-1H-1,2,3-triazol-1-yl)acetate

Participants

Reaction Steps

To a 100 ml round bottom flask equipped with a magnetic stir bar were added 2-{(S)-ammonio[1-(2-ethoxy-2-oxoethyl)-1H-1,2,3-triazol-4-yl]methyl}-5-(2,2,2-trifluoroethoxy)pyridinium dichloride (0.265 g, 0.614 mmol), 4-tert-butylphenylacetic acid (0.118 g, 0.614 mmol), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (0.118 g, 0.614 mmol), 1-hydroxy-7-azabenzotriazole (0.084 g, 0.614 mmol) and diisopropylethylamine (0.236 ml, 1.350 mmol) into 10.0 ml of CH2Cl2. The resulting solution was stirred at room temperature for 2 hours. The reaction mixture was diluted with water and the two layers were separated. The aqueous layer was extracted with CH2Cl2. The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and filtered. The filtrate was concentrated in vacuum. Purification by preparative HPLC (20-90% CH3CN/H2O over 30 min, 0.05% added TFA, C18 column) to afford 0.150 g (46%) of the title compound. 1H NMR (CDCl3, 400 MHz) δ 8.219 (s, 1H), 7.610 (m, 2H), 7.340 (m, 3H), 7.255 (m, 1H), 7.184 (m, 2H), 6.322 (d, J=7.96 Hz, 1H), 5.041 (s, 2H), 4.347 (q, J=7.97 Hz, 2H), 4.210 (q, J=7.14 Hz, 2H), 3.912 (br s, 1H), 3.585 (s, 2H), 1.278 (s, 9H), 1.249 (t, J=7.19 Hz, 3H). ES-MS [M+1]+: 534.3.

Provenance & Evidence