Hydrochloric Acid → HCID 56952891, compound

Participants

Reaction Steps

7.5 mg (8 μmol) of N-(3-carboxypropyl)-N-methyl-L-valyl-N-[(3R,4S,5S)-3-methoxy-1-{(2S)-2-[(1R,2R)-1-methoxy-3-{[(2S)-1-{[4-(methoxycarbonyl)benzyl]oxy}-3-phenylpropane-2-yl]amino}-2-methyl-3-oxopropyl]pyrrolidin-1-yl}-5-methyl-1-oxoheptan-4-yl]-N-methyl-L-valinamide (example 26) were dissolved in 0.25 ml of THF/water (1:1). 0.8 mg (32 μmol) of lithium hydroxide were added and the batch stirred for 3 hours at RT. Then, the reaction composition was acidized with 1 N hydrochloric acid and extracted three times with 5 ml of ethyl acetate each. The organic phases were dried over magnesium sulphate and evaporated in a vacuum. Following lyophilization from dioxane, the yield was 2.3 mg (71% purity, 22% o. th.) of the title compound in the form of a white solid substance.

Provenance & Evidence