反应详情

A solution of ethyl 4-(3-(2-((tert-butoxycarbonylamino)methyl) acrylamido)phenyl)-7H-pyrrolo[2,3-d]pyrimidine-5-carboxylate (50 mg, 0.11 mmol) in 4 M HCl in dioxane solution (537 μL, 2.15 mmol) was stirred for 1 hour. The reaction mixture was quenched with aqueous saturated sodium bicarbonate (500 μL) and extracted with ethyl acetate (×3). The combined organic layers were dried over sodium sulfate and concentrated under reduced pressure. The residue was purified by chromatography on silica eluting with 0-20% MeOH/DCM to give ethyl 4-(3-(2-(aminomethyl)acrylamido)phenyl)-7H-pyrrolo[2,3-d]pyrimidine-5-carboxylate. LRMS (ESI) calc'd for C19H20N5O3 [M+H]+: 366, found 366.

1. customThe reaction mixture was quenched with aqueous saturated sodium bicarbonate (500 μL)
2. extractionextracted with ethyl acetate (×3)
3. dry with materialThe combined organic layers were dried over sodium sulfate
4. concentrationconcentrated under reduced pressure
5. customThe residue was purified by chromatography on silica eluting with 0-20% MeOH/DCM