反应详情

The green filtrate was evaporated and purified by flash chromatography on Grade V neutral Alumina, eluting first with n-hexane to remove a fast running yellow band and then with dichloromethane to obtain the major blue/gray peak containing pheophytin-a. Treatment of pheophytin-a with 500 ml sulfuric acid in methanol for 12 hours at room temperature in the dark under nitrogen was followed by dilution with dichloromethane. The reaction mixture was rinsed with water and then 10% aqueous sodium bicarbonate and the organic layer was dried, evaporated, and the residue recrystallized from dichloromethane/methanol to obtain 1.8 gm methyl pheophorbide-a. Methyl pheophorbide-a appears to be inactive in the in vivo tumoricidal activity assay when injected at a dose of 5 mg/kg.

1. customThe green filtrate was evaporated
2. custompurified by flash chromatography on Grade V neutral Alumina
3. washeluting first with n-hexane
4. customto remove
5. customwith dichloromethane to obtain the major blue/gray peak
6. washThe reaction mixture was rinsed with water
7. dry with material10% aqueous sodium bicarbonate and the organic layer was dried
8. customevaporated
9. customthe residue recrystallized from dichloromethane/methanol