Trifluoroacetic acid → HCID 88496366, compound

Participants

Reaction Steps

A mixture of diphenylmethyl 7-[(Z)-2-(2-tritylaminothiazol-4-yl)-2-methoxyiminoacetamido]-3-[(E)-3-(5,6-dihydroxybenzimidazol-1-yl)-1-propen-1-yl]-3-cephem-4-carboxylate (XIn) (171 mg, 0.175 mmol), anisole (0.5 ml) and TFA (4 ml) was stirred for an hour at room temperature. The mixture was diluted with isopropyl ether to precipitate a crude product. The product was chromatographed on a column of Prep C18 (Waters, 20×300 mm), and the column was eluted successively with 10% MeOH/H2O, 15% MeOH/H2O and 25% MeOH/H2O. The eluate was monitored by UV absorption at 254 nm and the fractions showing UV absorption were further checked by HPLC.*The fractions containing the desired product were combined and concentrated in vacuo. The concentrate was freeze-dried to give 52 mg (52% yield) of the title product.

Provenance & Evidence