Dimethylformamide → 17alpha,19-Dihydroxy-6-methyl-4,6-pregnadiene-3,20-dione

Participants

Reaction Steps

Under the conditions of Example 1, 10 g. of megestrol (17α-hydroxy-6-methyl-4,6-pregnadiene-3,20-dione), dissolved in 400 ml. of dimethylformamide, is fermented for 48 hours with a Nigrospora sphaerica culture. After the batch has been worked up and the crude product crystallized from acetone/diisopropyl ether, 6.5 g. of 17α,19-dihydroxy-6-methyl-4,6-pregnadiene-3,20-dione is obtained, m.p. 184°-186° C.

Provenance & Evidence