反应详情

Methyl 2-{[(4-{2-[(2,4-difluorobenzyl)(heptyl)amino]-2-oxoethyl}phenyl)thio]methyl}-benzoate (31 mg, 0.057 mmol) was dissolved in THF (1 ml) and cooled in an ice-bath. Lithium hydroxide (2 mg, 0.075 mmol) in water (1 ml) was added. After the addition, the cooling bath was removed and the mixture was stirred overnight. LC-MS showed there was very little product. More lithium hydroxide (3 mg) was added and the mixture was stirred for 6 days more and HPLC showed about 30% product. More (3 mg) of lithium hydroxide was added and the mixture was stirred for 13 days more. It was evaporated in vacuum to remove THF. The residue was acidified with 10% hydrochloric acid, pH˜3, and extracted with ethyl acetate (×2). The organic phases were combined, dried (magnesium sulphate) and evaporated. Chromatography of the residue on a column (ISOLUTE® SI, 500 g/3 ml) using DCM, MeOH/DCM (0.5:99.5) as eluant gave 17 mg desired product, yield 56%.

1. temperaturecooled in an ice-bath
2. additionAfter the addition
3. customthe cooling bath was removed
4. additionMore lithium hydroxide (3 mg) was added
5. stirringthe mixture was stirred for 6 days more
6. additionMore (3 mg) of lithium hydroxide was added
7. stirringthe mixture was stirred for 13 days more
8. customIt was evaporated in vacuum
9. customto remove THF
10. extractionextracted with ethyl acetate (×2)
11. dry with materialdried (magnesium sulphate)
12. customevaporated