HCID 178448348, compound → HCID 178476271, compound

Participants

Reaction Steps

1 g of H-Arg-Lys(Boc)-Glu(OBut)-Val-Tyr(But)-OBut.2HCl (0.966 mmole) is dissolved in a mixture of 10 ml of trifluoroacetic acid and 1 ml of ethylmercaptan. The mixture is allowed to stand for one hour at room temperature and is concentrated. The residue is dissolved again in a little methanol and the solution is concentrated once more. The residue is dissolved in water, insoluble matter is filtered off and the solution is chromatographed over a strongly basic exchanger in the acetate form. The eluate is concentrated and purified further by being chromatographed on a hydroxypropylated crosslinked dextran gel in a water/acetic acid/n-butanol mixture. Yield 491 mg (57%), [α]D =-21.8° (c=1, water). Aminoacid analysis: Glu 1.00, Val 0.97, Tyr 0.92, Lys 1.03 and Arg 0.99. Protein content: 82%.

Provenance & Evidence