Sodium Chloride → (+-)-2-Hydroxyisocaproic acid

Participants

Reaction Conditions

Reaction Steps

220 parts by weight of this glucose was mixed with 12,100 parts by weight of a culture medium (10 g/l of peptone, 5 g/l of yeast extract, 2 g/l of meat extract, 5 g/l of NaCl, 2 g/l of cysteine hydrochloride and 5 g/l of calcium carbonate), and the mixture formed was injected into a pressure bottle. After the gaseous-phase portion in the bottle was displaced with nitrogen gas, the bottle was hermetically closed with a butyl rubber stopper, which was then treated in an autoclave (121° C., 98 kPa pressure, 10 minutes). Thereafter, the culture medium treated was cooled to 30° C., and 121 parts by weight of a pre-culture fluid of 2-hydroxyisocaproic acid productive strain HICA432 isolated in the manner as described previously was added thereto by means of a syringe when the bottle was kept hermetically stoppered, followed by a static culture at 30° C. for 3 days. Thereafter, the culture fluid was filtered, and the water and volatile components of the filtrate obtained were evaporated off under normal-temperature reduced pressure. Thereafter, the remaining solid content was heated under reduced pressure. The sublimated gas was cooled and the precipitated solid matter was collected to obtain 10 parts by weight of 2-hydroxyisocaproic acid.

Provenance & Evidence