Sodium Chloride → HCID 86587262, compound

Participants

Reaction Conditions

Reaction Steps

280 parts by weight of this glucose was mixed with 15,400 parts by weight of a culture medium (10 g/l of peptone, 5 g/l of yeast extract, 2 g/l of meat extract, 5 g/l of NaCl, 2 g/l of cysteine hydrochloride and 5 g/l of calcium carbonate), and the mixture formed was injected into a pressure bottle. After the gaseous-phase portion in the bottle was displaced with nitrogen gas, the bottle was hermetically closed with a butyl rubber stopper, which was then treated in an autoclave (121° C., 98 kPa pressure, 10 minutes). Thereafter, the culture medium treated was cooled to 30° C., and 154 parts by weight of a pre-culture fluid of 2-hydroxyisocaproic acid/phenyllactic acid productive strain HICA432 isolated in the manner as described previously was added thereto by means of a syringe when the bottle was kept hermetically stoppered, followed by a static culture at 30° C. for 3 days. Thereafter, the culture fluid was filtered, and the water and volatile components of the filtrate obtained were evaporated off under normal-temperature reduced pressure, followed by NMR analysis. As a result, it was ascertained that a mixture of 13 parts by weight of 2-hydroxyisocaproic acid and 16 parts by weight of phenyllactic acid was obtained.

Provenance & Evidence