Citric Acid → ethyl (3S)-3-hydroxy-3-{6-[(methylamino)carbonyl]-2-naphthyl}-3-(1-phenylsulfonyl-1H-imidazol-4-yl)propanoate

Temperature

-25 °C

The Reformatsky reagent was obtained according to the method described in Example 14. The obtained Reformatsky reagent (27.3 mL, corresponding to 2.5 eq.) was cooled to 0° C. or below, cinchonine (2.6 g, 8.9 mmol), pyridine (2.3 mL, 23.0 mmol) and THF (24 mL) were added thereto, and the mixture was stirred at the same temperature for 30 min. The reaction mixture was cooled to −25° C., N-methyl-6-((1-phenylsulfonyl-1H-imidazol-4-yl)carbonyl)-2-naphthamide (3.0 g, 7.2 mmol) was added thereto. The obtained reaction mixture was stirred at −20° C. for 1 hr. To the obtained reaction mixture was added the Reformatsky reagent (10.9 mL, corresponding to 1.0 eq.) at −25° C., and the mixture was stirred for 1.5 hr. The Reformatsky reagent (10.9 mL, corresponding to 1.0 eq.) was added again thereto −25° C., and the mixture was stirred for 1.5 hr. To the obtained reaction mixture were added ethyl acetate (42 mL) and 20 w/v % aqueous citric acid solution (42 mL) at 10° C. or below. The separated organic layer was washed successively with 10% sodium chloride-containing 20 w/v % aqueous citric acid solution (30 mL, twice) at 5° C., 5 w/v % aqueous sodium bicarbonate (30 mL, three times), and water (30 mL). The organic layer was concentrated to the volume of 18 mL under reduced pressure to give a residue. To the obtained residue was added methanol (30 mL), and the mixture was concentrated to the volume of 18 mL under reduced pressure to give a residue. To the residue was added methanol to adjust the volume to 30 mL. Water (3 mL) was added thereto at room temperature, and the mixture was stirred for 1 hr. Then water (21 mL) was added dropwise thereto at the same temperature, and the mixture was stirred. The obtained crystals were collected by filtration, and washed with a mixed solvent (9 mL) of methanol/water (1:3, volume ratio). The obtained wet crystals were dried under reduced pressure to give ethyl (3S)-3-hydroxy-3-{6-[(methylamino)carbonyl]-2-naphthyl}-3-(1-phenylsulfonyl-1H-imidazol-4-yl)propanoate (3.2 g, 6.3 mmol). yield 88%. enantiomeric excess: 61% ee.

1. customThe Reformatsky reagent was obtained
2. temperatureThe obtained Reformatsky reagent (27.3 mL, corresponding to 2.5 eq.) was cooled to 0° C. or below
3. customThe obtained reaction mixture
4. stirringwas stirred at −20° C. for 1 hr
5. customTo the obtained reaction mixture
6. additionwas added the Reformatsky reagent (10.9 mL, corresponding to 1.0 eq.) at −25° C.
7. stirringthe mixture was stirred for 1.5 hr
8. additionThe Reformatsky reagent (10.9 mL, corresponding to 1.0 eq.) was added again
9. stirring−25° C., and the mixture was stirred for 1.5 hr
10. customTo the obtained reaction mixture
11. washThe separated organic layer was washed successively with 10% sodium chloride-
12. additioncontaining 20 w/v % aqueous citric acid solution (30 mL
13. customat 5° C.
14. concentrationThe organic layer was concentrated to the volume of 18 mL under reduced pressure
15. customto give a residue
16. concentrationthe mixture was concentrated to the volume of 18 mL under reduced pressure
17. customto give a residue
18. stirringat room temperature, and the mixture was stirred for 1 hr
19. stirringat the same temperature, and the mixture was stirred
20. filtrationThe obtained crystals were collected by filtration
21. washwashed with a mixed solvent (9 mL) of methanol/water (1:3, volume ratio)
22. customThe obtained wet crystals were dried under reduced pressure

ORD record

ord-e6b30e593f194301931e49439b791da7

Source dataset

uspto-grants-2016_03

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US09278938B2