反应详情

To a mixture of 3-{[(benzyloxy)carbonyl]amino}-7-(3-oxomorpholin-4-yl)quinoline-2-carboxylic acid (0.014 g, 0.033 mmol), tert-butyl ((3R,4R,5S)-1-(3-aminopyridin-4-yl)-4-{[tert-butyl(dimethyl)silyl]oxy}-5-methylpiperidin-3-yl)carbamate (0.019 g, 0.043 mmol) and HATU (0.034 g, 0.089 mmol) in DMF (0.30 mL), DIPEA (0.019 mL, 0.11 mmol) was added. The mixture was stirred at room temperature for 2 h. The mixture was diluted with 5 mL of EtOAc and 3 mL of 1 M NaOH. The resulting layers were separated and the aqueous layer was extracted with EtOAc. The organic extracts were combined and concentrated under reduced pressure. To the resulting residue, 1 mL of 4 M HCl in dioxane and 1 mL of MeOH were added. The mixture was stirred at room temperature for 1 h, then concentrated. The resulting residue was purified by preparative HPLC (XBridge™ C18 column, eluting with a gradient of MeCN/water containing 0.1% NH4OH, at a flow rate of 60 mL/min.) to afford the sub-title compound. LCMS calc. for C33H36N7O6 [M+H]+: m/z=626.3. found: 626.3.

1. additionwas added
2. customThe resulting layers were separated
3. extractionthe aqueous layer was extracted with EtOAc
4. concentrationconcentrated under reduced pressure
5. additionTo the resulting residue, 1 mL of 4 M HCl in dioxane and 1 mL of MeOH were added
6. stirringThe mixture was stirred at room temperature for 1 h
7. concentrationconcentrated
8. customThe resulting residue was purified by preparative HPLC (XBridge™ C18 column
9. washeluting with a gradient of MeCN/water containing 0.1% NH4OH, at a flow rate of 60 mL/min.)