Water → HCID 118006196, compound

Participants

Reaction Conditions

Reaction Steps

A solution of isopropylmagnesium chloride-lithium chloride complex in tetrahydrofuran (1.3 M, 0.70 mL, 0.92 mmol) was added dropwise to a stirring mixture containing 3-(4-(1H-imidazol-1-yl)benzyl)-4-chloro-6-iodo-2-methoxyquinoline (350 mg, 0.736 mmol, Intermediate 17: step c) and 1-(4-benzoylpiperidin-1-yl)ethanone (170 mg, 0.736 mmol, Intermediate 18) in tetrahydrofuran (7.4 mL) at −35° C. (dry ice-acetone cooling bath). The cooling bath was allowed to slowly warm to 23° C. After 16 hours, water (30 mL) and ethyl acetate (30 mL) were added. The layers were separated. The aqueous layer was extracted with ethyl acetate (30 mL) and the organic layers were combined. The combined solution was dried with sodium sulfate and the dried solution was filtered. Silica gel (2 g) was added to the filtrate and the mixture was concentrated by rotary evaporation to afford a free-flowing powder. The powder was loaded onto a silica gel column for flash column chromatography purification. Elution with ethyl acetate initially, grading to 10% methanol-ethyl acetate provided the title compound as an impure off-white solid. The impure solid was further purified by RP-HPLC eluting initially with 5% acetonitrile-water (containing 0.05% trifluoroacetic acid), grading to 95% acetonitrile-water (containing 0.05% trifluoroacetic acid) to provide the title compound as a white solid after partitioning the twice purified material between dichloromethane-saturated aqueous sodium bicarbonate solution, separating the layers, drying the organic layer with sodium sulfate, filtering the dried solution, and concentrating the filtrate to dryness. MS (ESI): 581.1 [M+H]+.

Provenance & Evidence