Diisopropylethylamine → 2-fluoro-9-isopropyl-N-(1-methyl-1H-pyrazol-4-yl)-9H-purin-6-amine

関与物質

反応手順

To a solution of 6-chloro-2-fluoro-9-isopropyl-9H-purine (200 mg, 0.932 mmol), as prepared in step 1 of the alternate method of Example 2, in nBuOH, (4.66 mL) was added 1-methyl-1H-pyrazol-4-amine (109 mg, 1.12 mmol) and DIPEA (482 mg, 3.73 mmol) and the mixture stirred at ambient temperature for 6 hr to yield crude 2-fluoro-9-isopropyl-N-(1-methyl-1H-pyrazol-4-yl)-9H-purin-6-amine. (S)-3-(methylsulfonyl)-N-(pyrrolidin-3-yl)propanamide hydrochloride (289 mg, 1.12 mmol) was then added to the reaction mixture and heated at 100° C. for 16 hr. LCMS showed unreacted intermediate so the reaction was heated at 110° C. for another 24 hr. The reaction was then cooled to ambient temperature and potassium tert-butoxide (3.73 mL, 3.73 mmol) was added and the resulting mixture stirred at ambient temperature for 30 min. Water was added and the reaction was extracted with DCM (3×50 mL), then the aqueous layer was extracted with ethyl acetate (2×50 mL). The combined organic layers were dried with Na2SO4, concentrated, loaded onto silica and purified via flash chromatography using 0-20% EtOH/EtOAc to give the title compound (290 mg, 78% yield) as a pink solid, 1H NMR (400 MHz, DMSO-d6) δ ppm 9.56 (s, 1 H) 8.38 (d, J=6.72 Hz, 1 H) 7.97 (s, 1 H) 7.89 (s, 1 H) 7.75 (s, 1 H) 6.20-6.34 (m, 1 H) 6.05-6.18 (m, 1 H) 5.60 (dd, J=10.03, 2.32 Hz, 1 H) 4.56-4.73 (m, 1 H) 4.43 (br. s., 1 H) 3.76-3.92 (m, 4 H) 3.68 (d, J=5.14 Hz, 2 H) 3.43-3.51 (m, 1 H) 2.15-2.28 (m, 1 H) 1.87-1.99 (m, 1 H) 1.51 (d, J=6.85 Hz, 6 H). m/z for C19H25N9O 397.25 and 396.30 (M+H)+.

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