HCID 66723670, compound → HCID 56836122, compound

Participants

Reaction Conditions

Reaction Steps

(5-{[(5-Benzyl-1-methyl-1H-[1,2,4]triazole-3-carbonyl)-amino]-methyl}-4,6-dimethyl-pyridin-2-yl)-carbamic acid tert-butyl ester (29 mg, 0.064 mmol) was dissolved in a mixture of dichloromethane (4 ml) and TFA (1 ml). The reaction mixture was shaken at 23° C. for 18 h. Solvents were removed in vacuo. The residue was dissolved in dichloromethane/methanol 1:1 (1 ml) and purified over VARIAN SCX-SPE cartridge (strong cation exchange), washing with methanol and releasing the compound with 2M ammonia in methanol. The basic fraction was evaporated in vacuo to give the title compound. 1H-NMR (DMSO, 400 MHz) 8.5 (s, 1H), 7.2-7.3 (m, 5H), 7.0 (br s, 2H) 6.5 (s, 1H), 4.3 (d, 2H), 4.2 (s, 2H), 3.8 (s, 3H), 2.4 (s, 3H), 2.3 (s, 3H). UPLC-MS (method F) Rt=0.92 min, [M+H]+=351.4. HPLC (method G) Rt=1.281 min.

Provenance & Evidence