反应详情

A mixture of {4,6-Dimethyl-5-[({1-[4-(5-methyl-pyrazol-1-ylmethyl)-benzyl]-1H-[1,2,3]triazole-4-carbonyl}-amino)-methyl]-pyridin-2-yl}-carbamic acid tert-butyl ester (132 mg, 0.249 mmol) and 5 mL of TFA in 10 mL of DCM was stirred at room temperature for 2 h. The reaction mixture was concentrated under reduced pressure. The residue was purified by preparative HPLC (Waters SunFire Prep C18 OBD 5 μm 30×100 mm, Flow 40 mL/min, ACN/water (0.1% TFA) 5/95 over 2 min, then ACN/water (0.1% TFA) 5/95-100/0 over 15 min) to yield the title compound. 1H-NMR (DMSO-d6, 600 MHz) 8.59 (s, 1H), 8.29 (t, 1H), 7.33 (s, 1H), 7.28 (d, 2H), 7.09 (d, 2H), 6.09 (s, 1H), 6.05 (s, 1H), 5.63 (s, 2H), 5.59 (s, 2H), 5.26 (s, 2H), 4.32 (d, 2H), 2.29 (s, 3H), 2.17 (s, 3H), 2.16 (s, 3H); [M+H]+=431.0.

1. concentrationThe reaction mixture was concentrated under reduced pressure
2. customThe residue was purified by preparative HPLC (Waters SunFire Prep C18 OBD 5 μm 30×100 mm, Flow 40 mL/min, ACN/water (0.1% TFA) 5/95 over 2 min