反应详情

Tert-butyl 5-[4-(3-oxa-8-azabicyclo[3.2.1]octan-8-yl)-5-phenyl-thieno[2,3-d]pyrimidin-2-yl]oxy-2-azabicyclo[2.2.1]heptane-2-carboxylate (269 mg, 0.503 mmol) was dissolved in DCM (2 mL) and trifluoroacetic acid (2 mL). The mixture was stirred at room temperature for 2 hrs and then evaporated. The residue was dissolved in water (2 mL) and DCM (2 mL). Solid sodium bicarbonate was added to neutralize the remaining acid. The layers were separated and the aqueous was extracted with DCM (2×2 mL). The combined organic layers were evaporated to give a brown solid. This was purified by preparative HPLC (acidic method 1). Fractions containing the target compound (by LCMS) were combined and evaporated. The residue was dissolved in methanol and applied to an SCX cartridge (2 g, equilibrated with methanol). The cartridge was washed with methanol (2 column volumes) then 0.5 M ammonia in methanol (2 column volumes). The ammonia solution was evaporated to give 8-[2-(2-azabicyclo[2.2.1]heptan-5-yloxy)-5-phenyl-thieno[2,3-d]pyrimidin-4-yl]-3-oxa-8-azabicyclo[3.2.1]octane as a white solid (6 mg). LCMS RT=3.16 min. M+1=435.

1. customevaporated
2. dissolutionThe residue was dissolved in water (2 mL)
3. additionSolid sodium bicarbonate was added
4. customThe layers were separated
5. extractionthe aqueous was extracted with DCM (2×2 mL)
6. customThe combined organic layers were evaporated
7. customto give a brown solid
8. customThis was purified by preparative HPLC (acidic method 1)
9. additionFractions containing the target compound (by LCMS)
10. customevaporated
11. dissolutionThe residue was dissolved in methanol
12. washThe cartridge was washed with methanol (2 column volumes)
13. customThe ammonia solution was evaporated