Trifluoroacetic acid → HCID 90067328, compound

Participants

Reaction Steps

TFA (0.25 ml, 3.24 mmol) was added to a stirred solution of 3-[[(3R,3aR,6R,6aR)-6-[6-chloro-5-(4-phenylphenyl)-1-(2-trimethylsilylethoxymethyl)imidazo[4,5-b]pyridin-2-yl]oxy-2,3,3a,5,6,6a-hexahydrofuro[3,2-b]furan-3-yl]oxy]propane-1,2-diol (6.1 mg, 9.32 μmol) in DCM (0.25 ml). The reaction mixture was stirred at room temperature. After 2.5 hours, the reaction mixture was evaporated under reduced pressure. Potassium trimethylsilanolate (9.2 mg, 0.072 mmol) was added to a stirred solution of the residue in THF (0.5 ml). The reaction mixture was stirred at room temperature. After 1.5 hours, additional potassium trimethylsilanolate (18.2 mg, 0.142 mmol) was added to the reaction mixture. After an additional 1.5 hours, the reaction mixture was evaporated under reduced pressure to give a pale amber residue. This material was purified by preparative HPLC reverse phase (C-18), using a 30×150 mm Sunfire™ column and eluting with a 20%-100% acetonitrile/water+0.05% TFA gradient followed by a 100% acetonitrile+0.05% TFA flush. The product fractions were combined, evaporated under reduced pressure, and lyophilized from ethanol and benzene to give the title compound as a white solid. LC-MS: calculated for C27H26ClN3O6 523.15 observed m/e: 524.22 (M+H)+ (Rt 1.16/2 min); 1H NMR δ (ppm) (CD3OD): 7.98 (s, 1H), 7.74-7.78 (m, 4H), 7.71 (d, J=7.4 Hz, 2H), 7.48 (t, J=7.7 Hz, 2H), 7.39 (t, J=7.4 Hz, 1H), 5.59 (qt, J=5.1 Hz, 1H), 5.01 (t, J=5.3 Hz, 1H), 4.65 (t, J=5.0 Hz, 1H), 4.13-4.17 (m, 3H), 3.98-4.01 (m, 1H), 3.54-3.81 (m, 6H).

Provenance & Evidence