Trifluoroacetic acid → HCID 90067364, compound

Participants

Reaction Steps

Step B(b): TFA (0.1 mL) was added to a stirred mixture of 5-([1,1′-biphenyl]-4-yl)-N-((3aR,6R,6aS)-6-((tert-butyldimethylsilyl)oxy)hexahydrofuro[3,2-b]furan-3-yl)-6-chloro-1-((2-(trimethylsilyl)ethoxy)methyl)-1H-imidazo[4,5-b]pyridin-2-amine (major isomer) (20 mg, 0.029 mmol) in DCM (0.5 ml) and the mixture was stirred at room temperature overnight. The reaction mixture was concentrated under reduced pressure. The residue was purified by preparative HPLC Reverse phase (C-18), eluting with acetonitrile/water+0.1% TFA to give the title compound. LC-MS: calculated for C24H21ClN4O3 448.13 observed m/e: 449.15 (M+H)+ (Rt 1.18/2 min); 1H NMR δ (ppm) (CD3OD): 7.88-7.34 (m, 10H), 4.75 (m, 1H), 4.62 (m, 1H), 4.46 (m 1H), 4.37 (m, 1H), 4.28 (dd, 1H), 4.00 (dd, 1H), 3.81 (dd, 1H), 3.68 (dd, 1H).

Provenance & Evidence