Ethyl 2-[(3R,3aR,6R,6aS)-6-[6-chloro-5-(4-phenylphenyl)-1-(2-trimethylsilyl-ethoxy-methyl)imidazo[4,5-b]pyridin-2-yl]oxy-2,3,3a,5,6,6a-hexahydrofuro[3,2-b]furan-3-yl]acetate → Ethyl 2-[(3R,3aR,6R,6aS)-6-[[6-chloro-5-(4-phenylphenyl)-1H-imidazo[4,5-b]pyridin-2-yl]oxy]-2,3,3a,5,6,6a-hexahydrofuro[3,2-b]furan-3-yl]acetate

HRID 929221Notes

Reaction equation

HRID 929221 reaction equation
[Cl:1][C:2]1[CH:3]=[C:4]2[N:22](COCC[Si](C)(C)C)[C:21]([O:31][C@H:32]3[C@H:36]4[O:37][CH2:38][C@@H:39]([CH2:40][C:41]([O:43][CH2:44][CH3:45])=[O:42])[C@H:35]4[O:34][CH2:33]3)=[N:20][C:5]2=[N:6][C:7]=1[C:8]1[CH:13]=[CH:12][C:11]([C:14]2[CH:19]=[CH:18][CH:17]=[CH:16][CH:15]=2)=[CH:10][CH:9]=1>C(O)(C(F)(F)F)=O.C(Cl)Cl>[Cl:1][C:2]1[CH:3]=[C:4]2[NH:22][C:21]([O:31][C@H:32]3[C@H:36]4[O:37][CH2:38][C@@H:39]([CH2:40][C:41]([O:43][CH2:44][CH3:45])=[O:42])[C@H:35]4[O:34][CH2:33]3)=[N:20][C:5]2=[N:6][C:7]=1[C:8]1[CH:13]=[CH:12][C:11]([C:14]2[CH:15]=[CH:16][CH:17]=[CH:18][CH:19]=2)=[CH:10][CH:9]=1

Participants

4

Reactant 1

HCID 131735097Ethyl 2-[(3R,3aR,6R,6aS)-6-[6-chloro-5-(4-phenylphenyl)-1-(2-trimethylsilyl-ethoxy-methyl)imidazo[4,5-b]pyridin-2-yl]oxy-2,3,3a,5,6,6a-hexahydrofuro[3,2-b]furan-3-yl]acetate

Product 1

HCID 118413257Ethyl 2-[(3R,3aR,6R,6aS)-6-[[6-chloro-5-(4-phenylphenyl)-1H-imidazo[4,5-b]pyridin-2-yl]oxy]-2,3,3a,5,6,6a-hexahydrofuro[3,2-b]furan-3-yl]acetate

Reagents, catalysts & solvents 2

HCID 6344DichloromethaneSolventHCID 6422Trifluoroacetic acidSolvent

Procedure

A mixture of ethyl 2-[(3R,3aR,6R,6aS)-6-[6-chloro-5-(4-phenylphenyl)-1-(2-trimethylsilylethoxymethyl)-imidazo[4,5-b]pyridin-2-yl]oxy-2,3,3a,5,6,6a-hexahydrofuro[3,2-b]furan-3-yl]acetate (34 mg, 0.052 mmol) in TFA (0.5 mL) and DCM (0.5 mL) was stirred at room temperature. After 1 hour, the reaction mixture was evaporated to an oil, dissolved in EtOH (1 mL) and purified by preparative HPLC reverse phase (C-18), using a 30×150 mm Sunfire™ column and eluting with a 20%-100% acetonitrile/water+0.05% TFA gradient followed by a 100% acetonitrile/water+0.05% TFA flush. The desired fractions were lyophilized to give the title compound as a solid. LC-MS: calculated for C28H26ClN3O5 519.16 observed m/e: 520.22 (M+H)+ (Rt 0.74/2 min); 1H NMR δ (ppm) (CD3OD): 7.87 (s, 1H), 7.75 (ABq, 4H), 7.71 (d, 2H), 7.48 (dd, 2H), 7.38 (dd, 1H), 5.57 (qt, 1H), 5.01 (t, 1H), 4.60 (t, 1H), 4.16 (q, 2H), 4.10 (dd, 1H), 4.00-4.06 (m, 2H), 3.53 (dd, 1H), 2.71 (dd, 1H), 2.59 (m, 1H), 2.51 (dd, 1H) and 1.27 (t, 3H).

Workup

  1. customthe reaction mixture was evaporated to an oil
  2. dissolutiondissolved in EtOH (1 mL)
  3. custompurified by preparative HPLC reverse phase (C-18)
  4. washeluting with a 20%-100% acetonitrile/water+0.05% TFA
  5. customflush

Sources & evidence

ORD record
ord-487b5fe0747b4f1e8c792c3f7158880b
Source dataset
uspto-grants-2016_03
Patent number
US09290517B2

Reaction notes

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