反应详情

A round bottomed flask was charged with di-dansyl cystamine (100 mg, 0.16 mmol), TCEP (46 mg, 1 eq), and MeOH (10 ml). The reaction mixture was stirred at ambient temperature under argon for 3 hrs. Bromomaleimide (56 mg, 2 eq) in MeOH (5 ml), was added to the reaction mixture. After 16 hrs, the reaction mixture was concentrated in vacuo. The residue was worked up with DCM and brine. The organic layers were combined, dried (MgSO4) and purified by flash chromatography (silica gel, 0-30% EtOAC-CHCl3) to yield the desired compound (73 mg, 55%). 1HNMR (CDCl3, 600 MHz), δ8.5 (1H, d J 8.5 Hz aromatic H's), δ8.2 (2H, m aromatic H's), δ8.1 (1H, s CONH), δ7.5 (2H, m, aromatic H's), δ7.15 (1H, d, J=7.5 Hz aromatic H's), δ6.0 (1H, s, CO2CH) δ5.89 (1H, t, J 6.4 SO2NH), δ3.20 (2H, q, J 6.7 NHCH2), δ3.99 (2H, t, J 6.9 SCH2), δ2.86 (6H, s NCH3); 13CNMR (CDCl3, 150 MHz), 6169.6, 168.0, 152.0, 150.7, 134.4, 131.0, 129.9, 129.7, 129.5, 128.8, 123.4, 119.3, 118.7, 115.6, 45.5, 41.0, 31.8; IR (cm−1) 3277 (br) 1720 (s) MS (ES−) m/z relative intensity: 404 (M, 100); Exact mass calculated for [C18H18N3O4S2] requires m/z 404.0739. Found 404.0733.

1. waitAfter 16 hrs
2. concentrationthe reaction mixture was concentrated in vacuo
3. dry with materialdried (MgSO4)
4. custompurified by flash chromatography (silica gel, 0-30% EtOAC-CHCl3)