Sodium Hydroxide → HCID 67727693, compound

Participants

Reaction Steps

Cyclohexyl (S)-(+)-3-(3,4-dichlorobenzoylamino)-3-(4-(2-chlorophenyl)-2-ethyl-6H-thieno[3,2-f] [1,2,4]triazolo[4,3-a] [1,4]diazepin-9-yl)propionate (0.40 g) obtained in Example 270 was dissolved in methanol (5 ml). A 2N aqueous sodium hydroxide solution (0.6 ml) was added, and the mixture was stirred for 3 hours. The solvent was evaporated. Water was added and the mixture was washed with ethyl acetate. Citric acid was added to the aqueous layer to make the layer acidic. The mixture was extracted with ethyl acetate and the extract was dried over magnesium sulfate. The solvent was evaporated, and the residue was purified by silica gel column chromatography using a mixed solvent of chloroform, methanol and acetic acid as an eluent to give 96 mg of (S)-(+)-3-(3,4-dichlorobenzoylamino)-3-(4-(2-chlorophenyl)-2-ethyl-6H-thieno[3,2-f] [1,2,4]triazolo[4,3-a] [1,4]diazepin-9-yl)propionic acid.

Provenance & Evidence