反应详情

The totally protected phosphorothioate dinucleoside (3c) is dissolved in an acetic acid/water/methanol mixture (8:1:1, v:v:v) (5 ml) and left with stirring at ambient temperature all night. The following day, the reaction mixture is evaporated and coevaporated several times with water and with toluene. The residue is then chromatographed on a column of silica gel using a gradient of methanol (0 to 10%) as eluent. The fractions containing the detritylated phosphorothioate triester dinucleoside (4c) are collected and concentrated under reduced pressure. The residue is coevaporated with dioxane and then lyophilized in dioxane. (4c) is obtained in the form of a white powder (20 mg, 85%).

1. waitleft
2. customThe following day, the reaction mixture is evaporated
3. customThe residue is then chromatographed on a column of silica gel using a gradient of methanol (0 to 10%) as eluent
4. additionThe fractions containing the detritylated phosphorothioate triester dinucleoside (4c)
5. customare collected
6. concentrationconcentrated under reduced pressure