N6-(2,4-Dinitrophenyl)-L-lysine → HCID 44593781, compound

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Reaction Steps

Fmoc-Lys(Dnp) was prepared from Lys(Dnp) as follows. Fmoc N-hydroxysuccinimide ester (1.89 g; 5.60 mmol) was dissolved in 30 mL of dimethoxyethane and stored at 4° C. Lys(Dnp) (1.63 g; 4.67 mmol) was dissolved in 10 mL of 10% aqueous Na2CO3 and added slowly to the dimethoxyethane solution. The reaction proceeded for 2 hours at 4° C. and overnight at room temperature. The solution was filtered, and the filtrate acidified to a pH of about 3 with concentrated HCl. Dimethoxyethane was removed by heating the solution under reduced pressure. The solution was extracted with ethyl acetate, and the ethyl acetate layer reduced by heating under reduced pressure to an oil. The oil [Fmoc-Lys(Dnp)] was used without further purification.

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