2',4',5',7'-Tetrabromofluorescein → Thiazin Eosin

HRID 968467Notes

Reaction equation

HRID 968467 reaction equation
[S:1]1[CH:6]=[CH:5][CH:4]=[CH:3][NH:2]1.[CH:7]1[CH:8]=[CH:9][C:10]2[C:16]3([C:25]4[CH:26]=[C:27]([Br:32])[C:28]([OH:31])=[C:29]([Br:30])[C:24]=4[O:23][C:22]4[C:21]([Br:33])=[C:20]([OH:34])[C:19]([Br:35])=[CH:18][C:17]3=4)[O:15][C:13](=[O:14])[C:11]=2[CH:12]=1.CC1C(O)=C(C=O)C(COP(O)(O)=O)=CN=1.O>>[S:1]1[CH:6]=[CH:5][CH:4]=[CH:3][NH:2]1.[CH:7]1[CH:8]=[CH:9][C:10]2[C:16]3([C:17]4[CH:18]=[C:19]([Br:35])[C:20]([OH:34])=[C:21]([Br:33])[C:22]=4[O:23][C:24]4[C:29]([Br:30])=[C:28]([OH:31])[C:27]([Br:32])=[CH:26][C:25]3=4)[O:15][C:13](=[O:14])[C:11]=2[CH:12]=1

Participants

4

Reactant 3

HCID 270202',4',5',7'-Tetrabromofluorescein5×HCID 38882Pyridoxal 5'-phosphate monohydrate2×HCID 157892451,2-Thiazine5×

Product 1

HCID 86599381Thiazin Eosin

Procedure

With pump 46 operating to pump thiazin stain from reservoir 41 to solenoid valve S-4 and pump 47 operating to pump eosin stain from reservoir 42 through the normally open side of resting solenoid valve S-5 to solenoid valve S-4, operation of solenoid valve S-4 will control flow and mixing of the thiazin and eosin stains. Solenoid valve S-4 may be set to actuate on a 250 millisecond cycle as described above to blend the eosin stain and the thiazin stain in a desired ratio. Solenoid valves S-3, S-1, and S-5 act as control valves in resting position to block all flow of stain. Actuating solenoid valve S-3 allows flow of the alternating thiazin and eosin stains as controlled by solenoid valve S-4 through the normally closed side of solenoid valve S-3 to mixing chamber MC-1 and nozzle 16. The alternating thiazin and eosin stains mix as they flow through the lines and mixing chamber MC-1 so are a substantially homogeneously mixed concentrated staining solution when sprayed from nozzle 16 onto any slides rotating in the carousel past nozzle 16.

Workup

  1. customstain from reservoir 41 to solenoid valve
  2. customto solenoid valve S-4, operation of solenoid valve S-4 will control
  3. waitto actuate on a 250 millisecond
  4. customas controlled by solenoid valve
  5. additiona substantially homogeneously mixed
  6. concentrationconcentrated staining solution when

Sources & evidence

ORD record
ord-e7f163bf7e5942f2b824b3cbefd5d9ea
Source dataset
uspto-grants-2007_12
Patent number
US07303920B2

Reaction notes

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