Hydrochloric Acid → HCID 21943195, compound

Participants

Reaction Conditions

Reaction Steps

Compound 59 (0.12 g, 0.196 mmol) was dissolved in MeOH (6 mL) and 2 M aqueous NaOH (3 mL) was added. The reaction mixture was stirred at 45° C. for 2.5 h and cooled down to 0° C. The mixture was neutralized with 5 N HCl to pH 3. After evaporation of solvent, the residue was dried under high vacuum and dissolved in DMF. After filtration, filtrate was concentrated by evaporation to a small volume and the product was separated by C18 reverse phase HPLC using buffer A (1% TFA in water) and buffer B (1% TFA acetonitrile) from 10% buffer B to 70% buffer B to provide a white solid (83%). MS (ESI) 599.18, 597.18 (M+H+).

Provenance & Evidence