Hydrazine hydrate (1:1) → Ser(tBu)-Thr(tBu)-NH-NH2

Participants

Reaction Steps

4.253 g (7.4 mmols) of DPC-Ser(tBu)-Thr(tBu)-OMe in 18 ml of methanol are mixed with 5.55 ml (about 110 mmols) of hydrazine hydrate and left to stand for 10 hours at room temperature and 2 hours at 40° C. The reaction solution is taken up in 450 ml of ethyl acetate and washed four times with half-saturated sodium chloride solution. The solution is dried over sodium sulphate, concentrated to about 15 ml, and mixed with about 5 ml of petroleum ether. Overnight 3.17 g of a hydrazide of melting point 132°-134° C. crystallise out.

Provenance & Evidence