Hydrochloric Acid → HCID 131711569, compound

Participants

Reaction Steps

3.24 g of BOC-Met-Leu-Gly-OMe are dissolved in 13 ml of 3.8 N hydrogen chloride in ethyl acetate and allowed to stand for 30 minutes at 20° C. On adding 100 ml of petroleum ether the tripeptide ester hydrochloride is precipitated as a sticky mass, and the supernatant solution is decanted off. On triturating with 100 ml of peroxide-free ether at 0° C. a finely powdered product is obtained which is filtered off and dried to constant weight over potassium hydroxide at room temperature in a desiccator. The compound is chromatographically pure but amorphous and strongly hygroscopic. It shows the following Rf-values on silica gel: Rf 43 C = 0.48; Rf (toluene-acetone 1:1) = 0.35.

Provenance & Evidence