Chloroform → HCID 11508813, compound

Participants

Reaction Steps

The C-terminal tetrapeptide (ε-INOC)Lys-Asn-Phe-Phe-OMe.2HCl is prepared by reacting Phe-OMe hydrochloride with BOC-PheHSE, which reaction is conducted in a suitable organic solvent, such as chloroform or methylene chloride, overnight at a basic pH. Insoluble materials are separated and the organic solution evaporated to dryness and the residue recrystallized. The blocked dipeptide is treated with anhydrous hydrogen chloride in ethyl acetate, thereby cleaving the BOC substituent to form Phe-Phe-OMe hydrochloride.

Provenance & Evidence